anti epha2 af3035 antibodies (R&D Systems)
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Anti Epha2 Af3035 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+epha2+antibody/Human+EphA2+Antibody/pm41991671-190-12-18
Average 93 stars, based on 43 article reviews
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Magnetic Cell Separation:Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells Article Snippet: MACS and FACS Testis cells were collected from wild-type B6 mice (MACS for characterization), green mice (MACS for transplantation), the transgenic mouse line B6,129-TgR (ROSA26)26SOR (designated as ROSA26 mouse; Jackson Laboratory, Bar Harbor, ME) (FACS) by collagenase type II digestion, as described previously [ 20 ]. .. For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with Incubation:Article Title: Expression and functional analyses of ephrin type-A receptor 2 in mouse spermatogonial stem cells Article Snippet: MACS and FACS Testis cells were collected from wild-type B6 mice (MACS for characterization), green mice (MACS for transplantation), the transgenic mouse line B6,129-TgR (ROSA26)26SOR (designated as ROSA26 mouse; Jackson Laboratory, Bar Harbor, ME) (FACS) by collagenase type II digestion, as described previously [ 20 ]. .. For MACS, dissociated testis cells (2 × 10 7 for wild-type and green mice; 5–10 × 10 7 for ROSA26 mice) were incubated with Article Title: Antitumour activity and tolerability of an EphA2-targeted nanotherapeutic in multiple mouse models. Article Snippet: 1Merrimack Pharmaceuticals, Cambridge, MA, USA.. 2Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.. 3Cancer Center, Texas Tech University Health Sciences Center, Lubbock, TX, USA. Immunoprecipitation:Article Title: Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts Article Snippet: Cells were surface-labelled with 0.13 mg/ml sulpho-NHS-LC-biotin (ThermoFisher) for 30 min at 4°C, before quenching with 1 M Tris (pH 6.8) and lysing in 50 mM Tris (pH 7.5), 250 mM sodium chloride, 10 mM magnesium chloride, 5 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, and Complete Protease Inhibitor (Roche). .. Protein was either immunoprecipitated with Article Title: Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts. Article Snippet: Cells were surface-labelled with 0.13 mg/ml sulpho-NHS-LC-biotin (ThermoFisher) for 30 min at 4°C, before quenching with 1 M Tris (pH 6.8) and lysing in 50 mM Tris (pH 7.5), 250 mM sodium chloride, 10 mM magnesium chloride, 5 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, and Complete Protease Inhibitor (Roche). .. Protein was either immunoprecipitated with Western Blot:Article Title: Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts Article Snippet: Cells were surface-labelled with 0.13 mg/ml sulpho-NHS-LC-biotin (ThermoFisher) for 30 min at 4°C, before quenching with 1 M Tris (pH 6.8) and lysing in 50 mM Tris (pH 7.5), 250 mM sodium chloride, 10 mM magnesium chloride, 5 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, and Complete Protease Inhibitor (Roche). .. Protein was either immunoprecipitated with Article Title: Inhibition of EphA2 by syndecan-4 in wounded skin regulates clustering of fibroblasts. Article Snippet: Cells were surface-labelled with 0.13 mg/ml sulpho-NHS-LC-biotin (ThermoFisher) for 30 min at 4°C, before quenching with 1 M Tris (pH 6.8) and lysing in 50 mM Tris (pH 7.5), 250 mM sodium chloride, 10 mM magnesium chloride, 5 mM EGTA, 1% Triton X-100, 0.5% sodium deoxycholate, and Complete Protease Inhibitor (Roche). .. Protein was either immunoprecipitated with Membrane:Article Title: Effectiveness of anti-erythropoietin producing Hepatocellular receptor Type-A2 antibody in pancreatic cancer treatment Article Snippet: .. After the proteins were transferred to a polyvinylidene fluoride membrane, the membrane was detected using the 1 μg/ml Binding Assay:Article Title: Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast displayed tumor antigens Article Snippet: .. Specific binding to yeast displayed EphA2 and CD44 extracellular domains of the EphA2 natural ligand Ephrin A1, and antibodies to EphA2 and CD44 suggests that the domains are not only displayed but displayed in a form that can be specifically recognized by antibodies ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Display of antigen domains on the surface of yeast (a) The extracellular domain (ECD) of receptor EphA2 was displayed on yeast surface and recognized by Recombinant:Article Title: Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast displayed tumor antigens Article Snippet: .. Specific binding to yeast displayed EphA2 and CD44 extracellular domains of the EphA2 natural ligand Ephrin A1, and antibodies to EphA2 and CD44 suggests that the domains are not only displayed but displayed in a form that can be specifically recognized by antibodies ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Display of antigen domains on the surface of yeast (a) The extracellular domain (ECD) of receptor EphA2 was displayed on yeast surface and recognized by Flow Cytometry:Article Title: Internalizing cancer antibodies from phage libraries selected on tumor cells and yeast displayed tumor antigens Article Snippet: .. Specific binding to yeast displayed EphA2 and CD44 extracellular domains of the EphA2 natural ligand Ephrin A1, and antibodies to EphA2 and CD44 suggests that the domains are not only displayed but displayed in a form that can be specifically recognized by antibodies ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window caption a7 caption a8 Display of antigen domains on the surface of yeast (a) The extracellular domain (ECD) of receptor EphA2 was displayed on yeast surface and recognized by FACS:Article Title: Antitumour activity and tolerability of an EphA2-targeted nanotherapeutic in multiple mouse models. Article Snippet: 1Merrimack Pharmaceuticals, Cambridge, MA, USA.. 2Roswell Park Comprehensive Cancer Center, Buffalo, NY, USA.. 3Cancer Center, Texas Tech University Health Sciences Center, Lubbock, TX, USA. |


